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Guide – Neutralizing solutions

Environmental control

Environmental control has two main objectives: monitoring potential sources of contamination and verifying the effectiveness of the remediation process.

Microbiological analysis

Two methods of microbiological analysis are commonly used: microbial indicator tests and pathogen-specific tests.

Indicator tests monitor the level of general hygiene through the total number of bacteria, coliforms or fecal coliforms. Specific tests detect pathogenic microorganisms such as Listeria spp. 

For information on cleaning and sanitizing measures, it is recommended that samples be taken immediately before the start of production and immediately after the clean-up and sanitization are complete.  

In addition, to obtain information on microbiological environmental conditions during production, it is recommended that samples be taken during production. 

It is essential to ensure that the neutralizer chosen is effective against the disinfectants used. 

When sampling on a surface previously exposed to chemical germicide treatment, it is necessary to incorporate a neutralizing agent suitable for the medium to preserve the viability of the microbial cells. 

The recommended neutralizing solutions for microbiological quality monitoring in food plants are: 

  • Dey-Engley Neutralizing Broth (DE);
  • Neutralizing buffer (NE); 
  • Buffered peptone water (BPW); 
  • Letheen broth (LT). 

Guide for selecting a neutralizing solution

The following tables serve as a guide for selecting the most efficient neutralizing solution for the most common cleaning products. For quantitative analysis, it is also important to use a neutralizing agent that will not promote bacterial growth, such as a neutralizing buffer.  

In addition, microbiological analysis should be carried out as soon as possible after sampling to avoid losses due to the death of viable micro-organisms. 

 
The table contains a list of various classes of sanitizers neutralized by each of our solutions to help our customers choose according to their needs..

NEUTRALIZING AGENTS

SANITIZER CLASS

EXAMPLE OF BRANDS

D/E NEUTRALIZING BROTH

LETHEEN BROTH

NEUTRALIZING BUFFER

BUFFERED PEPTONE WATER

Glutaraldehydes

Maxicide Plus, Cidex, Biocide G30, Banicide, Glutacide Metricide Plus



Formaldehydes

Formalin


Chlorine, bromine and iodine

Household bleach, Clorox, IodoFive, Dispatch



Quaternary ammonium compounds (Quats/QACs)

Citrus Disinfectant, Coverage 256, Dustbane Vangard, Envirocide





Chlorhexidine

Aseptol, Savlon, Nolvasan



Phenols (LpH)

Lysol, Dettol, Pine-sol, Bi-Arrest, ProSpray



Alcohol

Ethanol, isopropyl alcohol



Peroxides

Hydrogen peroxide, Hyperox, Virox




Acetic and lactic acids




The use of trademarks in this document is for informational purposes only. No license to use these marks or any proprietary rights is granted to LABPLAS inc. No control, direct or indirect, is exercised over the characteristics, quality of the product, the service, the use, advertising or exposure of the brand by LABPLAS inc. and no warranty is provided as to its composition, efficacy, level of safety, toxicity, efficacy or any other attribute desired when used. Under no circumstances may LABPLAS inc. be held liable for any damages resulting from the use of these products.

The table below describes each of the neutralizing solutions offered with our Sani-Sponge and Sani-Stick products, along with the recommended use.

PRODUCTS

SOLUTIONS

RECOMMENDED USE

DESCRIPTION

Sani-Sponge;
(polyurethane or cellulose sponge)

Sani-Stick

(polyurethane or cellulose sponge)

Letheen broth

Non-food and food contact surfaces

Letheen broth was developed as a medium meant for neutralization of multiple compounds found in sanitizers. Its enzymatic digest and beef extract components allow easy bacterial growth for microorganisms detection.

Sani-Sponge;
(polyurethane or cellulose sponge)

Sani-Stick

(polyurethane or cellulose sponge)

Buffered Peptone Water

Carcasses, Non-food and food contact surfaces

Buffered Peptone Water is a non-selective, pre-enrichment medium used to help bacteria recover from food processing injuries.Its enzymatic digest and stable pH allow bacterial growth.

Sani-Sponge;
(polyurethane or cellulose sponge)

Sani-Stick

(polyurethane or cellulose sponge)

Neutralizing buffer

Non-food and food contact surfaces

Neutralizing buffer is used for inactivation of the bactericidal and bacteriostatic effect of disinfectants used on food and dairy equipment. This buffer is used to preserve microorganisms without promoting their growth.

Sani-Sponge;
(polyurethane or cellulose sponge)

Sani-Stick

(polyurethane or cellulose sponge)

D/E Neutralizing broth

Non-food and food contact surfaces

Dey-Engley Neutralizing broth is a solution inhibiting the activity of a wide range of disinfectants. It contains glucose, tryptone and yeast extract components which allow easy bacterial growth for microorganisms detection.

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